접착성 성분을 첨가한 실험용 불소바니쉬의 항균 지속성
Antibacterial sustainability of experimental fluoride varnish added with adhesive components
신성진, 손주리, 오승한, 배지명,
소속 상세정보
신성진 ( Shin Seong-JIn ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
손주리 ( Son Ju-Lee ) - Wonkwang Health Science University Department of Dental Hygiene
오승한 ( Oh Seung-Han ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
배지명 ( Bae Ji-Myung ) - Wonkwang University College of Dentistry Department of Dental Biomaterials
Abstract
본 연구는 불소바니쉬에 글루타르알데하이드와 하이드록시에틸 메타크릴산(HEMA)과 같은 접착성 성분을 혼합하였을때 Streptococcus mutans에 대한 항균의 지속성이 증가되는지를 평가하고자 하였다. 실험용 불소바니쉬(EX1)를 제작후, EX1군에 HEMA를 혼합(EX2), 글루타르알데하이드를 혼합(EX3), HEMA와 글루타르알데하이드를 혼합(EX4)하여접착성 성분이 들어간 불소바니쉬를 제작하였다. 직경 6 mm polyethylene terephthalate 필름 디스크에 각 실험군을5 μL씩 적용하였고, 증류수에 넣은 후 37℃ 진탕배양기에서 각각 1시간, 3시간, 8시간, 12시간, 1일, 5일, 10일동안보관하여 불소바니쉬가 용출되도록 하였다. 각 시간 별로 필름 디스크를 꺼내어 S. mutans균에 대한 한천배지시험을실시하여 항균력이 지속되는지 평가하였다. 모든 불소바니쉬는 10일까지 항균 능력을 보였고, EX3군과 EX4군은 8시간에서10일까지 유의성있게 높은 항균 능력을 보였으며, 10일에서는 EX2군과 유의성 있는 차이가 없었다. Glutaraldehyde와HEMA 혹은HEMA 첨가 시 불소바니쉬의 항균의 지속성을 증가시킬 수 있다.
The purpose of this study was to evaluate the sustainability of antibacterial effect against Streptococcus mutans of experimental fluoride varnish mixed with 2-hydroxyethyl methacrylate (HEMA) and/or glutaraldehyde. Experimental fluoride varnish (EX1) was fabricated. To make fluoride varnish with adhesive materials, EX1 was mixed with 5 wt% of glutaraldehyde (EX2), 35 wt% of HEMA (EX3) or both 5 wt% of glutaraldehyde and 35 wt% of HEMA (EX4). 5 μL of each experimental group was applied to 6 mm diameter polyethylene terephthalate film discs. Disks were stored in distilled water in a shaking incubator at 37℃ for 1 hour, 3 hours, 8 hours, 12 hours, 24 hours, 5 days and 10 days. The antibacterial activities against S. mutans were evaluated by agar diffusion test with the discs at each time. The antibacterial activities were sustained up to 10 days in all groups. The antibacterial activities of EX3 and EX4 were significantly higher than other groups from 8 hours to 10 days and there were no significant differences from EX2 at 10 days. HEMA with and without glutaraldehyde can be applied to fluoride varnish to increase the sustainability of antibacterial activities.
키워드
불소바니쉬; 항균의 지속성; 하이드록시에틸메타크릴산; 글루타르알데하이드
Fluoride varnish; Sustainability of antibacterial activity; 2-hydroxyethyl methacrylate (HEMA); Glutaraldehyde
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